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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP629912Yeast1mgQuote
EP629912E. coli1mgQuote
BP629912Baculovirus200ugQuote
MP629912Mammalian Cell200ugQuote

Protein Information

SpeciesPsychrobacter cryohalolentis (strain K5)
UniProt IDQ1Q929
Gene NamemiaB; Locus:Pcryo_2047
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-497
Expression Tag6xHis
Purity>90%
AA SequenceMSVTVFNPRIDDAAASDITAVTPVITALNELSSAQAPVEKAATKKVFVTTQGCQMNVYDS GKMLDVLGDSHGMEVTHNIDEADVLLMNTCSIREKAQEKVFSELGRWRKLKEKRPDLVIG VGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDQSHQQREIAPKNRIGTVDVSFPS IEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAAQG VREINLLGQNVNGYRGEKDDGSICRFAELLHYVSHVDGVERIRYTTSHPLEFTDDIIDAY AQLPELVSHLHLPVQSGSNAILAAMKRNHTIDVYINQINKLKAIRPDIHLSSDFIIGFPG ETDQDFQDTLNLAKELNFDHSYSFIYSKRPGTPAAELPDDVSFKTKKERLAEFQKVIIDS TLAKTHEMVGTTTRVLVEQVANRHPDCLIGTADNTRTVMFPYDVDKMDEMLGKIVSVRIT DFVSPHMVKGEIEAVLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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