Request QuoteCatalog Number: xP631928RKSSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP631928RKSYeast1mgQuote
EP631928RKSE. coli1mgQuote
BP631928RKSBaculovirus200ugQuote
MP631928RKSMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium leguminosarum bv. viciae (strain 3841)
UniProt IDQ1MMB6
Gene NamemiaB; Locus:RL0395
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-473
Expression Tag6xHis
Purity>90%
AA SequenceMTQDSALLQAPEAIPSESLRDGSNSRKVFIKTYGCQMNVYDSMRMSDALARDGYEPTEDM EVADLVLLNTCHIREKAAEKVYSALGRLREMKKKKAADGREMMIGVAGCVAQAEGEEILR RAPAVDVVIGPQTYHRLPEALRRAKQGQRVVDTEYAIEDKFEHLPIAESRRIRARGVTAF LTVQEGCDKFCTFCVVPYTRGSEVSRSVSQIVEEAEKLADSGVREITLLGQNVNAWHGAG SQGEAWSLGDLLYRLAEIPGLARLRYTTSHPRDMDDRLINAHRDLSALMPYLHLPVQSGS DRILKAMNRRHTAAEYLALIERIRTVRPDIALSGDFITGFPGETDEDFKDTLRLVEEVRY AQAFSFKYSTRPGTPGAELKDQVPEEIKAERLERLQMLLLKQQQEFAESCVGKEIDLLLE KPGRMPEQLIGRSPWLQSVNVDAKASQIGDIIKVRITGTGTNSLFAEHAEAAV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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