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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP601869Yeast1mgQuote
EP601869E. coli1mgQuote
BP601869Baculovirus200ugQuote
MP601869Mammalian Cell200ugQuote

Protein Information

SpeciesSyntrophomonas wolfei subsp. wolfei (strain Goettingen)
UniProt IDQ0AYB7
Gene NamemiaB; Locus:Swol_0972
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMGDKEKKPLKYRILTYGCQMNVRDSETIAGLLEGSGFNQAEDLSEADLIVFNTCSVRHSA ENKVYGKLGEIASLKKKRPELLIAFGGCMAQLPEVRQKLKKRGVDVVFGTHNIHELPYLI ARAKEKRSPVFEVWEKEGSIVEPLPSCRKPGLSAFVNIMFGCNNFCSYCIVPYTRGRERS RKADDIIRELEELAAAGYKEVTLLGQNVNSYGRGLGEKIEFADLLYRANSVAGIERIRFT TSHPKDVSDRLLQAIAECEKLCEHIHAPLQAGSNRILQRMNRNYSREHYLKLVERMRHYV PGVSITSDLIVGFPGETEEDFLETLDMVERVRFDAAFTFLYSQRSGTRAAELAEQIPLEE KKQRLERLNRRQYQIATEINQELQGSIQEVLVEGPSKTNPQKLTSRTRSNRIVIFSGGKD LIGRLINVKITEAKTFSLFGEIFNE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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