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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP639374Yeast1mgQuote
EP639374E. coli1mgQuote
BP639374Baculovirus200ugQuote
MP639374Mammalian Cell200ugQuote

Protein Information

SpeciesNeorickettsia sennetsu (strain Miyayama)
UniProt IDQ2GCU4
Gene NamemiaB; Locus:NSE_0832
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-471
Expression Tag6xHis
Purity>90%
AA SequenceMLLYLADVVIFSHAKVYMEKIEKKNNSLKKFHIKTYGCQMNVYDSEMIEKIVSGLGFTLS ERAEDADLIILNTCNIREKAAEKLYSELGQIRLLQKKKQERILIVVAGCVAQAEGEEIMR RAENVDVVVGPQSIHSLPELIAKVNRQSGKAIKMEFDPIEKFDYLAEETRKRRVPQSSAF LSIQEGCDKFCAFCVVPYTRGAEYSRSTEEVYREALSLTTKGVKEITLLGQNVNGYHGTL DSGNKVLNLGQLISRLGKIPSLKRIRYTTSHPVDMHKELYDAHANESKLMPFVHLPVQSG SDKILKQMNRKYTTADYLKIINEFQNARSDIAFSSDFIVGFPGESDDDFQQTLALIEQVN YAQCYSFKYSPRPGTPGATYPQISEETKNTRLQKLQQLLKEKQLEFNKKMIGKTVTVLFD KKHPDKISGRTEYMQQVFSDDSNLLDKIVTMRVEDASTFTLKCTAEDIIST
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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