Request QuoteCatalog Number: xP641586RKKSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP641586RKKYeast1mgQuote
EP641586RKKE. coli1mgQuote
BP641586RKKBaculovirus200ugQuote
MP641586RKKMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium etli (strain CFN 42 / ATCC 51251)
UniProt IDQ2KD88
Gene NamemiaB; Locus:RHE_CH00376
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-469
Expression Tag6xHis
Purity>90%
AA SequenceMTQDSALLKAPEQTLRDGSNSRKVFIKTYGCQMNVYDSTRMSDALARDGYEPTEDMEEAD LVLLNTCHIREKAAEKVYSALGRLREMKKKKAADGREMMIGVAGCVAQAEGEEILRRAPA VDVVIGPQTYHRLPEALRRAKEGQRVVDTEYAIEDKFEHLPVAESRKIRARGVTAFLTVQ EGCDKFCTFCVVPYTRGSEVSRPVSQIVEEAEKLVEGGVREITLLGQNVNAWHGAGPRGE AWSLGDLLYRLAEIPGLARLRYTTSHPRDMDDRLIAAHRDLRALMPYLHLPVQSGSDRIL KAMNRRHTAAEYLSLIERIRAVRPDIALSGDFITGFPGETDKDFEDTLRLVEEVRYAQAF SFKYSTRPGTPGAELKDQVPEEIKAERLERLQALLLKQQQEFAESCIGKEIDLLLEKPGR MPGQLIGRSPWLQSVNVDAKASQIGDIIKVRITGTGTNSLFAERAEAAV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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