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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP651521Yeast1mgQuote
EP651521E. coli1mgQuote
BP651521Baculovirus200ugQuote
MP651521Mammalian Cell200ugQuote

Protein Information

SpeciesSodalis glossinidius (strain morsitans)
UniProt IDQ2NUT9
Gene NamemiaB; Locus:SG0811
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLHIKTWGCQMNEYDSSKMADLLDSTHGYQLTDNPEEADVLLLNTCSIREKAQEKVF HQLGRWHMLKEARPELIIGVGGCVASQEGAHIRERANYVDIIFGPQTLHRLPEMINHVNG TRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPCDD ILFEIAQLADQGVREVNLLGQNVNAYRGASYDGGICTFAELLRLVAAIDGIDRIRYITSH PIEFTDDIIDVYRDTPELVSFVHLPVQSGSDRILTMMKRAHTALEYKSIIRRLRKARPDI QISSDFIIGFPGETQQDFEQTMQLIADVNFDMSFSFIYSARPGTPAADMVDNVSEEEKKQ RLHILQERITQQAMQYSRRMKGKVQRILVEGTSRKNVMELSGRTENNRVVNFEGAPSMIG KFVDVEIVDVYPNSLRGVLLRTEDQMTLRSHESTASVIARTRKENELGVGMYQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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