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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP643608Yeast1mgQuote
EP643608E. coli1mgQuote
BP643608Baculovirus200ugQuote
MP643608Mammalian Cell200ugQuote

Protein Information

SpeciesErythrobacter litoralis (strain HTCC2594)
UniProt IDQ2N950
Gene NamemiaB; Locus:ELI_08495
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMKPTHSPKTYRVKSFGCQMNVYDGERMAEMLDEKGIEPAPEGEDADLVVLNTCHIREKAV DKVYSDIGRLTKGKTQTKAPMIAVAGCVAQAEGEEIMARAPAVSMVVGPQAYHRLPGMID AAVAGKRSTDTDMPADAKFAALPKRRKSAPSAFLTIQEGCDKFCTYCVVPYTRGAEISRP FSALIDEAKKLVEAGAKEITLLGQNVSAWTGEDAKGRALGMAGLIRELAKDPDLKRVRYT TSHPADMDDELIATHGEVEKLMPYLHLPVQSGNDRVLKAMNRSHTAESYLRLLERFRAAR PDLALSGDFIVGFPGETEAEFEDTLKIVDEVRYAQAYSFKYSPRPGTPAATMERQVPKEV MDERLQRLQAALNRDQAAFNAGSVGRTCEVLVERTGKHPGQWLGKSPWLQSVWFDGDVAI GDLVQVELVEAGPNSLAGQLLETVAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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