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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP311564SIUYeast1mgQuote
EP311564SIUE. coli1mgQuote
BP311564SIUBaculovirus200ugQuote
MP311564SIUMammalian Cell200ugQuote

Protein Information

SpeciesSolibacter usitatus (strain Ellin6076)
UniProt IDQ01RU5
Gene NamemiaB; Locus:Acid_6704
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMGAVSGKTFYIETFGCQMNAHDSEKVVGTLLAEGYEQVATPEAAELVFYNTCSIRDKAEQ KVFNRLQNFKREGMKGKIFGVLGCVAQQEGEKIFDRAPHVSLVAGSASYTRLPEMLVQLE AGNRRVTGLSLDTEETFDTPFTRRDNPHRAYLTIIEGCDKACAYCVVPFTRGPERSRTSE SVMAEARGLTEKGYTEVQLLGQNVNSYRDPSPAGWDFATLLAKVAEIPGMRRVRYTTSHP RDFVRPIVDAMDANEAICDHIHLPVQSGSSKVLAAMDRLYTRDEYLRRIDWIKSAKRRYS LTTDIIIGFPGETDADFEQTLDLLDEVQYDSLFSFKYSPRPNTSALAMEDRIPEEEKQRR LLTLQEKQRAIQIRRNAEMIGSIQEVLVEGRNQALGQWIGRTTCNRTLNFSHPDTNGNEL VGKYLPVRVTRSGPNSLVGESAALV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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