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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP606525Yeast1mgQuote
EP606525E. coli1mgQuote
BP606525Baculovirus200ugQuote
MP606525Mammalian Cell200ugQuote

Protein Information

SpeciesHaemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt) )
UniProt IDQ0I3Z1
Gene NamemiaB; Locus:HS_1155
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTQKLHIKTWGCQMNEYDSSKMADLLLNTHGLELTDVPEEADVLLLNTCSIREKAQEKVF HQLGRWKELKKQNPSLVIGVGGCVASQEGEHIRSRAPYVDIVFGPQTLHRLPEMINQIRG GKSAVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDD VLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGGICSFAELLRLVASIDGIDRLRFTTSH PIEFTDDIIDVYADTPELVSFLHLPVQSGSDRVLNLMKRNHTALEYKSIIRKLKKVRPNI QISSDFIVGFPGETDQDFEDTMNLIAQVNFDMSFSFIYSARPGTPAADMPDDVTEEEKKQ RLYLLQQRINNQAAQFSRAMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFAGTPNMIG KFVDIKITDVYTNSLRGEVVRTEDEMGLRIVQSPQVVINRTRKEDELGVGHYQG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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