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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP604782Yeast1mgQuote
EP604782E. coli1mgQuote
BP604782Baculovirus200ugQuote
MP604782Mammalian Cell200ugQuote

Protein Information

SpeciesMesorhizobium sp. (strain BNC1)
UniProt IDQ11BD9
Gene NamemiaB; Locus:Meso_3919
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMEQNLTTERSETSSSRAGTASRTDKKVFVKTYGCQMNVYDSQRMADALAAEGYRATDVIE DADLVLLNTCHIREKAAEKVYSELGRIRVLKEERAKQGRETVVGVAGCVAQAEGREILRR APAVDLVIGPQTYHRLPSVVTRARAGEKIVETEYAVEDKFDHLPAPERTAVRSRGVTAFL TVQEGCDKFCTFCVVPYTRGAEVSRPVAQIVAEAERLAEAGVRELTLLGQNVNAWHGEGP DGREWGLGRLLFRLAEIPGLDRLRYTTSHPRDMDEELIAAHRDLIKLMPYLHLPVQAGSD RILKAMNRKHTAADYLRLIERIRAARPDIAMSGDFIVGFPGETDRDFEDTMRIVRDVNYA QAFSFKYSPRPGTPGADMHDQVPDAVKDERLQRLQALLAEQQRAFGESLVGTEIDLLLEK PGRQAGQLVGRSPWLQPVIVEENAGQIGDIVRVRITSSGGHSLFCEPAGTARVAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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