Request QuoteCatalog Number: xP354865MVHSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP354865MVHYeast1mgQuote
EP354865MVHE. coli1mgQuote
BP354865MVHBaculovirus200ugQuote
MP354865MVHMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium bovis
UniProt IDP67086
Gene NamemiaB; Locus:Mb2752c
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMVAHDAAAGVTGEGAGPPVRRAPARTYQVRTYGCQMNVHDSERLAGLLEAAGYRRATDGS EADVVVFNTCAVRENADNRLYGNLSHLAPRKRANPDMQIAVGGCLAQKDRDAVLRRAPWV DVVFGTHNIGSLPTLLERARHNKVAQVEIAEALQQFPSSLPSSRESAYAAWVSISVGCNN SCTFCIVPSLRGREVDRSPADILAEVRSLVNDGVLEVTLLGQNVNAYGVSFADPALPRNR GAFAELLRACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTRNVCPALHMPLQSGSDRILR AMRRSYRAERYLGIIERVRAAIPHAAITTDLIVGFPGETEEDFAATLDVVRRARFAAAFT FQYSKRPGTPAAQLDGQLPKAVVQERYERLIALQEQISLEANRALVGQAVEVLVATGEGR KDTVTARMSGRARDGRLVHFTAGQPRVRPGDVITTKVTEAAPHHLIADAGVLTHRRTRAG DAHTAGQPGRAVGLGMPGVGLPVSAAKPGGCR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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