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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP529136FOBYeast1mgQuote
EP529136FOBE. coli1mgQuote
BP529136FOBBaculovirus200ugQuote
MP529136FOBMammalian Cell200ugQuote

Protein Information

SpeciesStreptomyces coelicolor (strain ATCC BAA-471 / A3 (2) / M145)
UniProt IDO69963
Gene NamemiaB; Locus:SCO5787; ORFs:SC4H2.08
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-505
Expression Tag6xHis
Purity>90%
AA SequenceMSSIDRSQSAGGTRTYEVRTYGCQMNVHDSERLSGLLEDAGYVRAPEGADGDADVVVFNT CAVRENADNKLYGNLGHLAPKKASRPGMQIAVGGCLAQKDRDTIVKRAPWVDVVFGTHNI GKLPVLLERARVQEEAQVEIAESLEAFPSTLPTRRESAYAAWVSISVGCNNTCTFCIVPA LRGKEKDRRPGDILAEVEALVAEGVSEITLLGQNVNAYGSDIGDREAFSKLLRACGNIDG LERVRFTSPHPRDFTDDVIAAMAETPNAMPQLHMPLQSGSDPVLKAMRRSYRQERYLGII EKVRAAIPHAAITTDIIVGFPGETEEDFEQTLHVVREARFAQAFTFQYSKRPGTPAAEME NQIPKAVVQERYERLVALQEEISWDENKKQVGRTLELMVAEGEGRKDGATHRLSGRAPDN RLVHFTKPDQEVRPGDVVTVEITYAAPHHLLAEGAVLDVRRTRAGDAWEKRNTAEQAKPA GVLLGLPKIGVPEPQPVVASGCGCD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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