Request QuoteCatalog Number: xP463076AXISize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP463076AXIYeast1mgQuote
EP463076AXIE. coli1mgQuote
BP463076AXIBaculovirus200ugQuote
MP463076AXIMammalian Cell200ugQuote

Protein Information

SpeciesActinobacillus pleuropneumoniae serotype 7 (strain AP76)
UniProt IDB3GYA4
Gene NamemiaB; Locus:APP7_1376
Protein Name
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMAKLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFS QLGRWKNWKKDKPDLIIGVGGCVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGG DRAIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV LFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHP IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQ ISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKER LARLQQRINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFQGTPDMIGK FVDIKITDVYTNSLRGDVVRTEDEMGLRVVESAASVIARTRKEDDLGVGKYVVNL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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