Request QuoteCatalog Number: xP453088ENBSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP453088ENBYeast1mgQuote
EP453088ENBE. coli1mgQuote
BP453088ENBBaculovirus200ugQuote
MP453088ENBMammalian Cell200ugQuote

Protein Information

SpeciesErwinia tasmaniensis (strain DSM 17950 / Et1/99)
UniProt IDB2VBL4
Gene NamemiaB; Locus:ETA_23370
Protein Name
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLHIKTWGCQMNEYDSSKMADLLNSTHGYTLTEQAEDADVLLLNTCSIREKAQEKVF ALLGRWKKLKESNPDMIIGVGGCVASQEGAQIRQRASCVDIVFGPQTLHRLPEMINSVRG TRSPVVDVSFPEIEKFDRMPEPRADGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDD ILFEVAQLAAQGVREVNLLGQNVNAYRGETFDGGICSFAELLRLVAAIDGIDRIRFTTSH PIEFNDDIIDVYRDTPELVSFLHLPVQSGADRILTLMKRAHTALEYKAIIRKLLAARPNI QISSDFIIGFPGETQADFEQTMKLIGEINFDISYSFIYSARPGTPAADLPDDVSEDEKKQ RLYILQDRINQQTTAWSRRKLGTVQRILVEGTSRKNVMELSGRTECNRVVNFEGSPEHIG KFVDVEITDVYANSLRGMLLRGEHQMALRTLETPASVIERTRKENELGVATWLP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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