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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP481106DKIYeast1mgQuote
EP481106DKIE. coli1mgQuote
BP481106DKIBaculovirus200ugQuote
MP481106DKIMammalian Cell200ugQuote

Protein Information

SpeciesDictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
UniProt IDB5YE40
Gene NamemiaB; Locus:DICTH_0940
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-440
Expression Tag6xHis
Purity>90%
AA SequenceMPKYYIITYGCQMNKSDSEKVAGILENLGYTPSEKMEEADIILLNTCSVRERAEEKVFGK LGELRKLKKRNQNLLIGIFGCMAQRMKEELIEKFPHVDFVLGSYKFTELPKILESLDGKK KVVLAEDIPSPQDVDFRVIKRENKFQAWIPIIYGCNNFCTYCIVPYLRGREKSRDPEEII REVEYLASQGVVEVTLLGQNVDSYGKDLGNVDLADLLVEIHRIPRIKRIRFLTSHPRDVS DKLIRVVATHPKVCPHWHLPLQAGSDRILRRMGRGYTYSEYKALIEKIRAEIPKASFSTD IIVGFPGEEEEDFLATRRALEEIKFDTVNLAIYSKRPGTPAANYEDLIPYETKKRWFDEL ENLQRKIIYEKNLSRVGKEEIVLAEEVNPKNPRELSGRTENYRLVFFEAEKELIGKFLLV KITEARLWSLKGEVIREVDL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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