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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP469036DWRYeast1mgQuote
EP469036DWRE. coli1mgQuote
BP469036DWRBaculovirus200ugQuote
MP469036DWRMammalian Cell200ugQuote

Protein Information

SpeciesCoprothermobacter proteolyticus (strain ATCC 35245 / DSM 5265 / BT)
UniProt IDB5Y8R7
Gene NamemiaB; Locus:COPRO5265_0825
Protein Name
Region Expressed1-426
Expression Tag6xHis
Purity>90%
AA SequenceMKYYIFTYGCQMNKNDSEMVSGILKSSGWEEAKNVVDSDLVVINTCSVRLHAEERAIGTI SALKKLGKKVVVMGCMSEVRGNEIMSRFPHVQAVLGPSYEAHILDVLNGERRILVGDEKV DFEKYSSANRKEKHSVYVSIMKGCDDFCTYCIVPFTRGRVQSRDPESILEEVRVCVDNGA VEITLLGQNVNDYGKDLSGWDFVSLVERVATIDGVRRIRFMSPHPANFKKDDITRLANLP QVAPYYHLPLQSGDDEILRRMNRKYTTGEFAELVGFIRESVPNVAIGTDLIVGFPGESDE HFQNTFKFLEKMQFDVVYMAIYSPRPGTAAARQETSFVPAEVAKARYDELLRLQEKISYS INQRYVGTLQEVLIDREDKTTGKFIGRTPTNKTVVFTSIRHVSPGEFVDVRINEAKSWVL YGEAAD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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