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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP481637HUYYeast1mgQuote
EP481637HUYE. coli1mgQuote
BP481637HUYBaculovirus200ugQuote
MP481637HUYMammalian Cell200ugQuote

Protein Information

SpeciesHelicobacter pylori (strain P12)
UniProt IDB6JKJ8
Gene NamemiaB; Locus:HPP12_0268
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-437
Expression Tag6xHis
Purity>90%
AA SequenceMKVYIETMGCAMNSRDSEHLLSELSKLDYKETSDPKMADLILINTCSVREKPERKLFSEI GQFAKIKKPNAKIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHKEKAVEVAI DYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILKEAEKLA NNGTKELMLLGQNVNNYGVRFSSEHAKVDFSDLLDKLSEIPGIERIRFTSPHPLHMNDGF LERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGISTDIIV GFPNESDKDFEDTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSSSRLERLQNR HKEILEEKAKLEVGKTHVVLVENRREMDNQIVGFEGRSDTGKFIEVTCKEKRNPGELVKV EIISHSKGRLIAAIKGN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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