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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP469555OEDYeast1mgQuote
EP469555OEDE. coli1mgQuote
BP469555OEDBaculovirus200ugQuote
MP469555OEDMammalian Cell200ugQuote

Protein Information

SpeciesOligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5)
UniProt IDB6JCT6
Gene NamemiaB; Locus:OCAR_4521, OCA5_c00160
Protein Name
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMIMTPPRKLHIKSYGCQMNVYDAQRMVDTLAPEGFVETADPAEADLVILNTCHIREKASE KVFSELGRLRVIKEEAARTGREMKIAVAGCVAQAEGAEITRRASTVDVVVGPQSYHNLPK LLAKARDGAPAIETEFPIEDKFSALPAPKPAAIRARGVSAFVTVQEGCDKFCTFCVVPYT RGMEVSRPVADIVADAKRLAENGVRELTLIGQNVNAYHGDDENGRTSSLGRLLHRLAMLP GIARLRYSTSHPRDVDDSLIEAHRDIPAVMPFVHLPVQSGSDRILAAMNRKHTAADYIRT IERFRKVRPEIAFSSDFIVGFPGESEQDFADTLALVTQIGYAGAYSFKYSPRPGTPAADM QETDTALAVPAAVMDERLAQLQALIDAQQASFNRAAIGRTVEVLFERAAREPGQIVGRTA YLQPAHVMASPDIIGKVLPVTIDSLERYSLKGRLASEETAVASLRSTQRESETFA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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