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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP481565DXMYeast1mgQuote
EP481565DXME. coli1mgQuote
BP481565DXMBaculovirus200ugQuote
MP481565DXMMammalian Cell200ugQuote

Protein Information

SpeciesCoxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212) )
UniProt IDB6J1A4
Gene NamemiaB; Locus:CbuG_1430
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-439
Expression Tag6xHis
Purity>90%
AA SequenceMKKLYLKTHGCQMNEYDSAKMADVLKFSHGLELTEDPAVADVFLLNTCSVREKAQTKVFS ELGRWRPFKEKRPHVVIGVGGCVASQEGETILKQAPFVDIVFGPQTLHRLPDLLDSVIQK RKSVVDITFPEIEKFDRLPQPRAEGPSAFVSIMEGCSKYCTFCVVPYTRGEEISRPFDDV IAEVASLCEQGVREITLLGQNVNDYRGLMHDGQVADLALLIHYLAAMDNIERIRFTTSHP SAFSENLIDAYAEEPKLANHLHLPVQSGSDRILAAMKRNYTVLEYKSKIRKLRAVRPDIS LSSDFIIGFPGETDADFEATMNLIHDMGFDHSFSFIYSPRPGTPAAQLPDDVPMAVKKER LAILQNRINAKAAEISQSMVGTQQRILVTGPSKKYPDQLSGRTENNRVVNFNGDTPLIGQ MVTIKIKEARPYSLWGEIC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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