Request QuoteCatalog Number: xP542841DXDSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP542841DXDYeast1mgQuote
EP542841DXDE. coli1mgQuote
BP542841DXDBaculovirus200ugQuote
MP542841DXDMammalian Cell200ugQuote

Protein Information

SpeciesCorynebacterium urealyticum (strain ATCC 43042 / DSM 7109)
UniProt IDB1VDD8
Gene NamemiaB; Locus:cu0876
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-540
Expression Tag6xHis
Purity>90%
AA SequenceMCRAVAGVTRFVFTLALGVGSAHVTHAMKKKTSVSEQPEQRTYEVRTFGCQMNVHDSERL SGLLEDSGYQPAANGEEPDVLVFNTCAVRENADNRLYGTLAMVKPMKDRNPGMQIAVGGC MAQKDKDAVVDRAPWVDVVFGTHNIGSLPTLLERSAHNQRAEVEILDSLEEFPSVLPAKR ESAYSGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGEILAEVQALVDQGVQEVTLLGQNV NAYGVNFADEDLPRDRGAFAKLLRACGEIEGLERLRFTSPHPAEFTDDVIDAMAETPNVC PQLHMPLQSGSDRILKEMRRSYRSKKFLGILDKVRERIPHAAITTDIIVGFPGETEEDFQ ATLDVVEKARFSCAFTFQYSPRPGTPAATMPDQIPKAVVQERYERLIALQERIQAEDNKE LVGTTQELLVQETGGRKDAQRHRMSGRARDGRLVHFTPSEDVRPGDIVEVTITDARPFFL IADGPLVNHRLTKAGDMSGAGQTPTTAPIGVSLGVPTIGIKPQADATAAPKNVHEGCGCD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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