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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP460791HUZYeast1mgQuote
EP460791HUZE. coli1mgQuote
BP460791HUZBaculovirus200ugQuote
MP460791HUZMammalian Cell200ugQuote

Protein Information

SpeciesHelicobacter pylori (strain Shi470)
UniProt IDB2USA0
Gene NamemiaB; Locus:HPSH_01400
Protein Name
Region Expressed1-437
Expression Tag6xHis
Purity>90%
AA SequenceMKVYIETMGCAMNSRDSEHLLSELSKLDYKETSDPKAADLILINTCSVREKPERKLFSEI GQFAKIKKPNAKIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHKEKAVEVAI DYDESAYAFEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILKEAEKLA SNGTKELMLLGQNVNNYGARFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPLHMNDAF LERFAKNPKVCKSIHMPLQSGSSTVLKMMRRGYSKEWFLNRVEKLKALVPEVGISTDIIV GFPNESDKDFEDTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSSSRLERLQNR HKEILEEKAKLEVGKTHVVLVENRREIDNQIVGFEGRSDTGKFIEVACKEKRNPGELVRV EIVSHSKGRLIATAKGD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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