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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP485734MSYYeast1mgQuote
EP485734MSYE. coli1mgQuote
BP485734MSYBaculovirus200ugQuote
MP485734MSYMammalian Cell200ugQuote

Protein Information

SpeciesMethylobacterium chloromethanicum (strain CM4 / NCIMB 13688)
UniProt IDB7KVE0
Gene NamemiaB; Locus:Mchl_3528
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMKKAYVKSYGCQMNAYDAGRMADVLAAEGYSATDTVEEADVVVLNTCHIREKAAEKVYSE LGRLRVLKGDRAESGQETRIVVAGCVAQAEGREILSRAPAVDVVVGPQSYHRLPDLLRQS RETRVVDTEFPAEDKFDHLPARRNRGVTGFLTVQEGCDKFCAFCVVPYTRGAEVSRSVAA VVEEARRLVEGGVREITLIGQNVNAYHGDGPDGAPATLGQLMDALSAVPGLLRLRYTTSH PNDFADDLIAAHATNPLVMPYLHLPVQSGSDRILHAMNRRHTGDAYRRLIERIRNARPDI ALSSDFIVGFPGETDADFAETLRLVSDIGFSAAFSFKYSPRAGTPAAEREDAVPEAVKTE RLAALQDLLDRQRHAYNAASVGTLTEILVEKTGRHPGQVAGKTPHLQAVQFDAPASTIGT VVPVRITRAGSNSLFGEALEGAAAAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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