Request QuoteCatalog Number: xP451266BTASize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP451266BTAYeast1mgQuote
EP451266BTAE. coli1mgQuote
BP451266BTABaculovirus200ugQuote
MP451266BTAMammalian Cell200ugQuote

Protein Information

SpeciesBifidobacterium longum (strain DJO10A)
UniProt IDB3DQX6
Gene NamemiaB; Locus:BLD_0276
Protein Name
Region Expressed1-484
Expression Tag6xHis
Purity>90%
AA SequenceMNEDMMTEAERASIAADGTDGLLGKRGKGVFHIHTLGCQMNVHDSERIAGVLEANGYVPA TEDQINDNDLDLLVLNTCAVRENAAERMYGTIGRFNRVKLVRPNLQIAVGGCMAQLDRKK IADTAPWVSAVFGTKNIEDLPKLLDQNRATGKAQVQVTEQLRQFPSQLPAARASRISSWV AISVGCNNTCTFCIVPTTRGKEKDRRPGDILDEIRQCVADGAKEVTLLGQNVNSFGYGIG DRYAFSKLLRACGTIDGLERVRFTSPHPAAFTDDVIAAMAETPNIMHQLHFPLQSGSDRI LRAMRRSYRSAKFLDILGRIRAAMPDAQISTDIIVGFPGETEEDFQQTMDVVRQARFSSA FTFIYSPRPGTPAAAMEQIPRDVVQDRFDRLVALQEQITEENLATFEGRDVEVMITGKLG KKDTDTHRVTGREKTGVLVHIGVPEGEPVPEIGDFVTATVTHAGRHNLLADPDVAAGQTY SVRH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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