Request QuoteCatalog Number: xP477193GFNSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP477193GFNYeast1mgQuote
EP477193GFNE. coli1mgQuote
BP477193GFNBaculovirus200ugQuote
MP477193GFNMammalian Cell200ugQuote

Protein Information

SpeciesGeobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622)
UniProt IDB5EE49
Gene NamemiaB; Locus:Gbem_0761
Protein Name
Region Expressed1-441
Expression Tag6xHis
Purity>90%
AA SequenceMNQAKKLYLETFGCQMNVSDSEKIVTLMKGMGYQQTQDPVDADLVLLNTCSIRATAEQRV YGHLGKFKSIKKTKPGLIIGVGGCVAQQEGEKLLKKAPFVNLVFGTHNLHLLQGMVAAAE EGKRSSQTDFLDDEKRFDLFPHSEAEGGVTRFVTVMQGCDNFCAYCIVPHVRGREISRSA AKVVEEVRALADSGVTEVTLLGQNVNSYCSKQPGEPDFPDLLRLVAQVDGIERIRFTTSH PKDMSPRLIECFADLPKLAPHIHLPAQSGSDRVLERMNRGYTAQQYLAKVAALKEACPAI QFTGDMIVGFPGEDEAAFQDTMALMEQVQYADLFSFIYSARPGTKAAEYADDATRAEKQG RLERLQAAQKKTTLARNRSLEGTVQKVLVEGLSSTGDSLFGRTGGNRGTVMAGDPSLAGR VLDVKIVEGLQTLLKGEIVHD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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