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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP479383BYTYeast1mgQuote
EP479383BYTE. coli1mgQuote
BP479383BYTBaculovirus200ugQuote
MP479383BYTMammalian Cell200ugQuote

Protein Information

SpeciesAnaeromyxobacter sp. (strain K)
UniProt IDB4UK35
Gene NamemiaB; Locus:AnaeK_1514
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-464
Expression Tag6xHis
Purity>90%
AA SequenceMSDLVPLSRKPAPAAGGPAPSPAAPPRKVYVHTFGCQMNESDSDRMVELLGRHAYARAAS ADEADLILLNTCAVREKAEQKLLSALGRYREVKARRGALIAVSGCVAQQEKDRLLARVPY VDFVFGPDNIGRLPEMVERARDERFAETGWMDSEEYVFPRADPEAARGRVTAFVTAMKGC DNVCAFCIVPHTRGREVSRPFPDVVAECAALAGVGVREVTLIGQNVNSYTGGCTFADLLR RVAAVPGIDRIRFTTSHPHDLSDALVEVFRDEPKVMPHFHLPVQSGSDAVLRRMRRDYSV AEYLDRFDRLRAARPGIAITTDFIVGFPGETDADFEASLALLERARFEQSFSFVFSPRPH TVAAVRLGSAPEWRDVPREAAVARLERLLAAQRRIAGEILAGELGKVVEVLVEGPSDEPG ERLGRTPENRVVHLAADEAAAPAGARVAVRITRAGGSSLSGTLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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