Request QuoteCatalog Number: xP452494NHQSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP452494NHQYeast1mgQuote
EP452494NHQE. coli1mgQuote
BP452494NHQBaculovirus200ugQuote
MP452494NHQMammalian Cell200ugQuote

Protein Information

SpeciesNostoc punctiforme (strain ATCC 29133 / PCC 73102)
UniProt IDB2IT24
Gene NamemiaB; Locus:Npun_R0775
Protein Name
Region Expressed1-454
Expression Tag6xHis
Purity>90%
AA SequenceMTTSKRHYHITTFGCQMNKADSERMAGVLEDMGFEWSEDPNNADVILYNTCTIRDNAEQK VYSYLGRQAKRKHDQPDLTLIVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESV FDGNQVVATESVHIIEDITQPRRDSKVTAWVNVIYGCNERCTYCVVPNVRGIEQSRTPSA IRAEMEELGRQGYKEITLLGQNIDAYGRDLPGTTPEGRHLHNFTDLLYYVHDVPGIERLR FATSHPRYFTERLIKACAELPKVCKHFHIPFQSGDNELLKAMARGYTHEKYRRIIDTIRR YMPDASISADAIVGFPGETEAQFENTLKLVEDIGFDMLNTAAYSPRPGTPAALWDNQLSE EVKSDRLQRLNHLGNLKVAERSQRYFGRIEEVLVEDQNPKDQTQVMGRTDGNRLTFFSGD IKELKGQLVKVKITEVRPFSLTGQPVEVRQAIPV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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