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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP463667EXMYeast1mgQuote
EP463667EXME. coli1mgQuote
BP463667EXMBaculovirus200ugQuote
MP463667EXMMammalian Cell200ugQuote

Protein Information

SpeciesPolynucleobacter necessarius (strain STIR1)
UniProt IDB1XS06
Gene NamemiaB; Locus:Pnec_1563
Protein Name
Region Expressed1-448
Expression Tag6xHis
Purity>90%
AA SequenceMKKLYIKTFGCQMNEYDSGKMADLLHADEGMVMTDRPEDADVVLLNTCSIREKAEDKVFS DLGRLRELKKTKPNLLIGVGGCVASQEGQQIVSHAPYVDVVFGPQTLHRLSDLIAKRRET GLFQVDISFPEIEKFDHLPASRQTRGSAYVSIMEGCSKYCSYCVVPYTRGEEVSRSFDDV LTEVAGLASKGVKEIVLLGQNVNAYLGKMGDRQEIADFALLIEYIVEIPGVERIRFTTSH PKEFTQRLIDVYTKVPKLVSHLHLPVQHGSDAMLSAMKRGYTALEFKSIIRKMRAVRPDL TLSSDFIVGFPGETEADFEKLLKMVEELNFDNSFCFIFSPRPGTPAANLHDDTPYEVKLK RLQTLLALVESQANQISQKMLGNIERVLIEGLAKDGINLQGRAENNRVIHFTAPAQNIEN LIGQMVDIRITEVLNYTLRGKLVEAYVH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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