Request QuoteCatalog Number: xP479118SWSSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP479118SWSYeast1mgQuote
EP479118SWSE. coli1mgQuote
BP479118SWSBaculovirus200ugQuote
MP479118SWSMammalian Cell200ugQuote

Protein Information

SpeciesSalmonella newport (strain SL254)
UniProt IDB4SYN0
Gene NamemiaB; Locus:SNSL254_A0729
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVF HQLGRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSH PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDI QISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMIG KFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENELGVGFYQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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