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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP470290FLYYeast1mgQuote
EP470290FLYE. coli1mgQuote
BP470290FLYBaculovirus200ugQuote
MP470290FLYMammalian Cell200ugQuote

Protein Information

SpeciesStenotrophomonas maltophilia (strain R551-3)
UniProt IDB4SQK7
Gene NamemiaB; Locus:Smal_1357
Protein Name
Region Expressed1-472
Expression Tag6xHis
Purity>90%
AA SequenceMTGTPDVFPPATPGGTPLVALPAGPRKPDQVKGKLYIKTHGCQMNEYDSAKMADVLAASD GLELTDSPDDADVILVNTCSIREKAQEKVFSQLGVWKSLKNKGREVIIGVGGCVASQEGE AIIKRAPFVDLVFGPQTLHRLPELIRARREQKRPQVDISFPEIEKFDRLPEPRADGASAF VSIMEGCSKYCSFCVVPYTRGTEVSRPFEDVVVEVAQLAAQGVREINLLGQNVNAYRGPY GDGEFADLGLLIRTIAEIDGVGRIRFTTSHPLEFSDSLIDAFRDVPQLANFLHLPVQAGS DRVLSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETDADFEKTMKLIEDIGF DHSFSFIYSRRPGTPAADLEDTISDAEKHARLSRLQERINAHAAGISEKMVGTVQTVLVE GPSRKNPNELTGKTENMRSVNFPAPARLIGQFVDVVITEALTNSLRARVVAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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