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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP450276FLXYeast1mgQuote
EP450276FLXE. coli1mgQuote
BP450276FLXBaculovirus200ugQuote
MP450276FLXMammalian Cell200ugQuote

Protein Information

SpeciesStenotrophomonas maltophilia (strain K279a)
UniProt IDB2FJ92
Gene NamemiaB; Locus:Smlt1600
Protein Name
Region Expressed1-472
Expression Tag6xHis
Purity>90%
AA SequenceMTGTPDVFPPATPGGAPLVALPAGPRKPDQVKGKLYIKTHGCQMNEYDSAKMADVLAASD GLELTDTPEDADVILVNTCSIREKAQEKVFSQLGVWKGLKNKGREVIIGVGGCVASQEGE AIIKRAPFVDLVFGPQTLHRLPELIRARREQKRPQVDISFPEIEKFDRLPEPRAEGASAF VSIMEGCSKYCSFCVVPYTRGTEVSRPFEDVVVEVAQLAAQGVREINLLGQNVNAYRGPY GDGEFADLGLLIRTIAEIDGVGRIRFTTSHPLEFSDSLIDAFRDVPQLANFLHLPVQAGS DRVLSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETDADFEKTMKLIEDIGF DHSFSFIYSRRPGTPAADLEDTISDAEKHARLSRLQERINAHAAGISEKMVGTVQTVLVE GPSRKNPNELTGKTENMRSVNFPAPARLIGQFVDVVITEALTNSLRARVVAE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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