Request QuoteCatalog Number: xP476694ETBSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP476694ETBYeast1mgQuote
EP476694ETBE. coli1mgQuote
BP476694ETBBaculovirus200ugQuote
MP476694ETBMammalian Cell200ugQuote

Protein Information

SpeciesPelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1)
UniProt IDB4SCB6
Gene NamemiaB; Locus:Ppha_0367
Protein Name
Region Expressed1-440
Expression Tag6xHis
Purity>90%
AA SequenceMTTGGKNTFYIHTFGCQMNQADSATITSLLQQAGYVAAESEDRAGIILLNTCAVRENAVD RIEHYLQHLQGLKKRDKRLIVGILGCIPQHQREEMFATSPAIDLLAGPDTYRTLPQLIEQ ARSGAKPFSLDFNVAETYEGIDPVRQGSISAFVPVMRGCNNMCAYCVVPFTRGRERSHPF RAVMGEVQKLVASGYSEITLLGQNVNSYDDPEQGVNFAALLDAVSCAAPQARVRFTTSHP KDISGELVRTIANRPNICNHIHLPVQSGSTATLGRMNRGHTIEEYLEKIALIRSLLPGVT LSTDIIAGFCGEQEEDHQASLELLSTLRFDSAYMFYYSTRPGTFAARTLVDDVPEVVKKR RLQEIIDLQNTISGELFQQAIGSVVEVLAESESKRSAEQLMGRTPGNRAVVFDREGYRPG DLVRVLITAATSATLTGRPV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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