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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP476650AZXYeast1mgQuote
EP476650AZXE. coli1mgQuote
BP476650AZXBaculovirus200ugQuote
MP476650AZXMammalian Cell200ugQuote

Protein Information

SpeciesAlteromonas macleodii (strain DSM 17117 / Deep ecotype)
UniProt IDB4S0R8
Gene NamemiaB; Locus:MADE_1007155
Protein Name
Region Expressed1-477
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLYIKTWGCQMNEYDSEKMADLLDSTHGFSAAQSAEEADVILLNTCSIREKAQEKVF HQLGRWKTLKQDKPELIIGVGGCVASQEGDTIRQRAPFVDLVFGPQTLHRLPEMINELKG GAKSVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCTFCVVPYTRGEEVSRPVDD VLLEIAQLAGQGVREVNLLGQNVNAYRGENYDGTICRFSELLELVAAIDGIDRIRYTTSH PVEFTDDIIDAYASIPELVDHLHLPVQSGSDRILNLMKRGHTALEYKSKMRKLKKIRPNI SLSSDFIIGFPGETDADFEATMDLIQAVDYDLSFSFIYSARPGTPAADAVDDVTEETKKQ RLHLLQQRITQQALRIARHMVGTEQRILVEGPSKKNPMELSGRTENNRVVNFEGTPDMIG EFVDVNITDVFTNSLRGEVVRRESEMGLRVAVSPQSIMAKHQADMPDELGVGQFSPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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