Request QuoteCatalog Number: xP458684DUGSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP458684DUGYeast1mgQuote
EP458684DUGE. coli1mgQuote
BP458684DUGBaculovirus200ugQuote
MP458684DUGMammalian Cell200ugQuote

Protein Information

SpeciesClostridium botulinum (strain Eklund 17B / Type B)
UniProt IDB2TIA8
Gene NamemiaB; Locus:CLL_A1808
Protein Name
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMIKFDDKQLNNIVEQDGDKHFFIETWGCQMNEEDSEKLSGMLKSQGYEETENRDEASIVI FNTCCVRENAENKVFGNLGRLKNQKEKNPNLIIALCGCMMQQKGMADEILSRFPYVDIIF GTHNAYKFPEYLHRVQVEGVQVKEIFDKETEIVEGVPIDRKSNVKAFVTIMYGCNNFCTY CVVPYVRGRERSRRPEDIENEIKELVSSGYKEITLLGQNVNSYGKGLEEEITFAQLLRRI NEIDGLERLRFMTSHPKDLTLDVVYAIRDCDKLCEQIHLPVQSGSNEILQKMNRHYNKEQ YLELAKKIREEIPDVTFSTDIIVGFPGETEEDFEETINLVKEVRYDAAFTFIYSRRNHTP ADKMENQIPDDVKHDRFNRLVAAVNEGIVVGNKAAEGKIYEVLVEGTSKNNENKLTGRTR NAKLVNFDGCKEMIGKLVKVKIIEAKSFSLVGEVVE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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