Request QuoteCatalog Number: xP450853BXUSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP450853BXUYeast1mgQuote
EP450853BXUE. coli1mgQuote
BP450853BXUBaculovirus200ugQuote
MP450853BXUMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia phytofirmans (strain DSM 17436 / PsJN)
UniProt IDB2SYI5
Gene NamemiaB; Locus:Bphyt_3329
Protein Name
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMTKKVYVKTFGCQMNEYDSDKMVDVLGAAEGLVKTDTPEDADVILFNTCSVREKAQEKVF SDLGRVRELKEANPNLIIGVGGCVASQEGAAIVSRAPYVDLVFGPQTLHRLPQMIDKRRE SGRAQVDISFPEIEKFDHLPPARVDGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPLDD VLTEIAGLADQGVREVTLLGQNVNAYRAGLTLGSTEIADFAQLIEYVADIPGIERIRYTT SHPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP DLSLSTDMIVGFPGETEEDFDKMMALVHEMKYDTSFSFIYSPRPGTPAANLHDDTPREVK LKRLQHLQATIEENVQRISDSMVGKIERILVERPARKDPNELAGRTENNRVVNFPAPIAS HARLIGQMVDVKIVKAYPHSLRGELVLVHDDAPATTH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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