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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB 1

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB 1 can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP458166MVSYeast1mgQuote
EP458166MVSE. coli1mgQuote
BP458166MVSBaculovirus200ugQuote
MP458166MVSMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium marinum (strain ATCC BAA-535 / M)
UniProt IDB2HGN2
Gene NamemiaB1; Locus:MMAR_1566
Protein Name
Region Expressed1-529
Expression Tag6xHis
Purity>90%
AA SequenceMTQDHIVTERPPATRNDTAGNATARTYEVRTLGCLMNAHDSERMAGLLEDAGYIKADPGA PADLVVFNTCAVRENADNKLYGSLAHLAPIKASRPGMQIAVGGCLAQKDRHIVLDRAPWV DVVFGTHNIGSLPVLLERSRHNQDAQVEILESLRTFPSALPAARDSAYAAWVSISVGCNN SCTFCIVPSLRGKEADRRPGDILAEVQALVEQGVLEVTLLGQNVNSYGVNFADPDPSTGE EPLPRDRGAFAQLLRACGRIEGLERIRFTSPHPAEFTDDVILAMAETPAVCPHLHMPLQS GSDQILKAMRRSYRRDRYLGIIDKVRAAIPHAAITTDIIVGFPGETEHDFEQTLDVVQKA RFTSAFTFQYSPRPGTPAADMPDQIPKNVVQQRFERLVALQERISLESNRSLVGTRQELL VVAGEGRKNATTARISGRARDGRLVHFRPDSADGSVRPGDLVDVEITDAAPHHLIADGPL LQHRRTPAGDASERGQTPTTRGVGLGVPRITVAQAQIPSSGTHPDTACR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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