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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP538684DQRYeast1mgQuote
EP538684DQRE. coli1mgQuote
BP538684DQRBaculovirus200ugQuote
MP538684DQRMammalian Cell200ugQuote

Protein Information

SpeciesCaulobacter sp. (strain K31)
UniProt IDB0T155
Gene NamemiaB; Locus:Caul_0021
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMSETPQKRLYIKTYGCQMNVYDSERMADVLRPLGYGIVDEPEGADLVVLNTCHIREKATE KVYSELGYIKQMKGRKAEAGGQMTVAVAGCVAQAEGQEIMRRQPAVDLVVGPQAYHQLPE LIARAHRATGERLSADFAADEKFDALPAERQVSGVSAFLTVQEGCDKFCTFCVVPYTRGG EWSRPPEQIEDEARRLADQGVREVTLLGQNVNAYDGGGYTLARLVRRLAKIPGLDRIRYT TSHPRDMGDDLIEAHGELPELMPYLHLPVQAGSDKILKAMNRDHTAESYIRLIEKIRQAR PDIAMSGDFIVGFPGERDGDFEKTLDLVREVGFASAFSFKYSRRPGTPASAMPGQVDEAV KAERLERLNQLLDEQQKAFNILQVGKTMPVLFEKKGRNPGQIVGRSPYLQAVHAVGSEDL LGQIVPVRIESSAKMSLGGVLETQPLPEPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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