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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP418277PYEYeast1mgQuote
EP418277PYEE. coli1mgQuote
BP418277PYEBaculovirus200ugQuote
MP418277PYEMammalian Cell200ugQuote

Protein Information

SpeciesParvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966)
UniProt IDA7HZ82
Gene NamemiaB; Locus:Plav_3617
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMPEAGPEAEPGSPRKKIFVKTYGCQMNVYDSARMVDVMAPSGYTEVDAPEDADIVILNTC HIREKAAEKVYSELGRLRELKKEKSGRGEELLIGVAGCVAQAEGEEMRRRAPVVDLVLGP QTYHRLPEYVARLANGGPGIVETEFPVDDKFASLPMAEKRKTLARGATAFLTIQEGCDKF CTFCVVPYTRGSEFSRPVARILDEARSLVDAGVREITLLGQNVNAWHGEDQAGRPATLGY LIRALAEIEGLARLRYTTSHPRDMDEELIAAHRDVPALMPYLHLPVQSGSDRILAAMNRR HDADSYHRIIGRIRAAKPDIALSSDFIVGFPGETEADFEATLDLIRTVGFAQAYSFKYSP RPGTPAATEEDQVPEEVKSERLQRLQALLGEQQLAFNAGCAGRTMPVLFDRRGRGESQLV GRSPYLQSVHIDDAPEHLFGSLVDVVIEEGHRNSLRGRLREEAAVLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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