Request QuoteCatalog Number: xP415550FDSSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP415550FDSYeast1mgQuote
EP415550FDSE. coli1mgQuote
BP415550FDSBaculovirus200ugQuote
MP415550FDSMammalian Cell200ugQuote

Protein Information

SpeciesFervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)
UniProt IDA7HK86
Gene NamemiaB; Locus:Fnod_0454
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-430
Expression Tag6xHis
Purity>90%
AA SequenceMKVHIFTYGCQMNENDSEIVKQLLKDEGIELTDDENDADVVILNTCAVRKKSEEKVYSHI GKLRKKGKKIGIMGCVAEKEKENLFKRGVSFVIGTRALTKVPDAVLNAKNGNKQIYLEDT LDEIEYHKVETRSSNHHAWVTIIHGCDRFCTYCIVPYTRGREKSRPIDEVLKEVESLSQR GYKEFTFLGQNVDAYGKDLRDGTSLAKLLKLASQIDNVKRLWFLTSYPTDFSLEIPKVML ESEKVARSIHLPVQHGSDKILKAMNRRYTRQEYIDLINEIRKIVPDASISSDIIVGFPGE NDEDFEATVELVKLLKFERLNLAVYSPREGTVAWKYLKDDVPYQVKVRRMSYLLNLQKTI NKELNKSYLGKEVEVIVEAQAKNGLFYGRDIRNKIISFEANPESIGKNVIVKVNKISAGP LYGEIKKILD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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