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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP417508AUTYeast1mgQuote
EP417508AUTE. coli1mgQuote
BP417508AUTBaculovirus200ugQuote
MP417508AUTMammalian Cell200ugQuote

Protein Information

SpeciesAnaeromyxobacter sp. (strain Fw109-5)
UniProt IDA7HAH8
Gene NamemiaB; Locus:Anae109_1518
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-460
Expression Tag6xHis
Purity>90%
AA SequenceMTDLVSLSPKPASAPGTRPAAARKVYVHTFGCQMNASDSDRMIELLGRHAFARAETPDDA DLILLNTCAVREKAEQKLLSALGRYREVKARRGALIAVSGCVAQQEKDRLLARVPYVDFV FGPDNIGKLPEMVARAERERFAETGWMDSQDYVFPQADPEAARGRPTAFVTAMKGCDNVC AFCIVPHTRGREVSRAFPEIVAECASLAEVGVREVTLIGQNVNSYAGGCTFAELLRRVAA VPGIARIRFTTSHPHDLSDALVAVFRDEPKVMPHFHLPVQSGSDAVLARMRRDYTVAEYL DRFDRLRAARPGIAITTDFIVGFPGEGEADFEGSLALLERARFEQSFSFLFSPRPKTVAN LRLGTAPEWQEIPRAVAVERLERLQAAQRRIAAAALAAELGKVVEVLVEGASDEPGERLG RTPENRVVHLAADEAGAPTGALVRARITRAGGSSLSGTPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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