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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP535165AWPYeast1mgQuote
EP535165AWPE. coli1mgQuote
BP535165AWPBaculovirus200ugQuote
MP535165AWPMammalian Cell200ugQuote

Protein Information

SpeciesAcinetobacter baumannii (strain AYE)
UniProt IDB0V6R4
Gene NamemiaB; Locus:ABAYE0462
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-483
Expression Tag6xHis
Purity>90%
AA SequenceMTVQTFIPNGAKAASENTVTQPTHTTDVSIKKLYIETQGCQMNEYDSHRMADLLGDSHGY VLTNNPNEADILLMNTCSIREKAQEKVFSELGRWRKLKEQNPDLVIGVGGCVASQEGDNI QKRAPYVDMIFGPQTLHRLPQMLDQHHAQVEKPKKEKIKLVDISFPDIEKFDFLPEPRVE GFKAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDVLAEIAGLAEKGVREISLLGQNVNG YRGETFEGGICTFPELLRLVAEIPGIGRLRYTTSHPLEFSDELIQCYEDLPQMVSHLHLP VQSGSNDVLKAMKRNHTIDVYIDKIAKLRKIRPDMHLSSDFIIGFPGETDENFAETLQFI KDLDFDHSYSFVYSKRPGTPASDLPDTTPEHVKKERLAQVQQVIKQSSIEKTDAMLGKIE RVLIEKVSDQDPNILVGTADNTRLVTFVGDASWVGRFAEIEITEIKTLNLVYGELLNLEP DVA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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