Request QuoteCatalog Number: xP407735STVSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP407735STVYeast1mgQuote
EP407735STVE. coli1mgQuote
BP407735STVBaculovirus200ugQuote
MP407735STVMammalian Cell200ugQuote

Protein Information

SpeciesSinorhizobium medicae (strain WSM419) (Ensifer medicae)
UniProt IDA6U5H0
Gene NamemiaB; Locus:Smed_0040
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-472
Expression Tag6xHis
Purity>90%
AA SequenceMTQETALLPTSPEAGDLNVPARKVFVKTYGCQMNVYDSDRMSDALSRDGYVATDVLEEAD FVLLNTCHIREKAAEKVYSELGRLRDLKKAKAREGREMLIGVAGCVAQAEGKEILRRAPA VDLVIGPQTYHRLPEALRRARTGERIVETDYAIEDKFVHLPAPDKAKTRARGVTAFLTVQ EGCDKFCTFCVVPYTRGSEVSRPVAQIVAEAEKLVEGGVREITLLGQNVNAWHGAGPHGR EWGLGDLLRRLGEIEGLARLRYTTSHPRDMDDSLIEAHRSMAKLMPYLHLPVQSGSDRIL KAMNRRHTAAEYLALVARIRAVQPDLALSGDFIVGFPGETDEDFEDTLRLVDAVNYAQAF SFKYSTRPGTPGAELEQQVPEDVKAKRLEILQALLIKQQRGFAEACVGREIDLLLEKPGR MPGQLVGRSPWLQPVNVDAKASQIGDIIRVRITKAGPNSLFAEMIGETEARS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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