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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP430378STEYeast1mgQuote
EP430378STEE. coli1mgQuote
BP430378STEBaculovirus200ugQuote
MP430378STEMammalian Cell200ugQuote

Protein Information

SpeciesSalmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)
UniProt IDA9MKB7
Gene NamemiaB; Locus:SARI_02272
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVF HQLGRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSH PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDI QISSDFIVGFPGETSDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ RLYILQERINQQAMAWSRRMLGSTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMIG KFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENELGVGFYQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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