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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP430279NEWYeast1mgQuote
EP430279NEWE. coli1mgQuote
BP430279NEWBaculovirus200ugQuote
MP430279NEWMammalian Cell200ugQuote

Protein Information

SpeciesNeisseria meningitidis serogroup C (strain 053442)
UniProt IDA9M1G4
Gene NamemiaB; Locus:NMCC_0355
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVF SDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKET SGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKYCSFCVVPYTRGEEFSRPLND VLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDL CLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSFVFIYSPRPGTPAANLHDDTPHEEKVR RLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQLQARTANNRVVNFTGTPNMIN QMIDLEITEAYTFSLRGKVVEA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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