Request QuoteCatalog Number: xP417201VEASize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP417201VEAYeast1mgQuote
EP417201VEAE. coli1mgQuote
BP417201VEABaculovirus200ugQuote
MP417201VEAMammalian Cell200ugQuote

Protein Information

SpeciesVibrio harveyi (strain ATCC BAA-1116 / BB120)
UniProt IDA7N1G9
Gene NamemiaB; Locus:VIBHAR_01230
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEEPEEADVLLLNTCSIREKAQEKVF HQLGRWKTLKDKKPGVVIGVGGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPEMIKQSQS DEAPVMDISFPEIEKFDRLPEPRAEGATAFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDD VLFEIAQLADQGVREVNLLGQNVNAYRGPMHDGEICSFAELLRLVASIDGIDRIRFTTSH PLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRVLTMMKRPHTGIEYKSIIRKLRKARPDI QISSDFIVGFPGETDKDFQDTMKLIKDVDFDMSFSFIFSPRPGTPAADYPCDLSEQVKKE RLYELQQTVNTQAMRYSRQMLDTEQRVLVEGPSKKNLMELRARTENNRVVNFEGSADLIG QFVDVKITDVFANSLRGELVRTEKDMDLRSVISPTQMMAKTRREDELGVATFTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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