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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP416346XAVYeast1mgQuote
EP416346XAVE. coli1mgQuote
BP416346XAVBaculovirus200ugQuote
MP416346XAVMammalian Cell200ugQuote

Protein Information

SpeciesXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
UniProt IDA7IGB2
Gene NamemiaB; Locus:Xaut_1810
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-470
Expression Tag6xHis
Purity>90%
AA SequenceMHEPRKLYVKSFGCQMNVYDSHRMADTLAREGFVETQDPAEADLVILNTCHIREKAAEKV YSELGRLRKQKQEAGSDTMIAVAGCVAQAEGAEIMRRAPVVDLVVGPQSYHRLPELLAEA KAGKRVVDTEFPAEDKFDHLPAPTRAATRSRGPAAFVTVQEGCDKFCTFCVVPYTRGAEV SRPVAKIMDEAARLVDQGVREISLIGQNVNAFHGAGPDGTTWSLARLMEHMARLDGLARL RYTTSHPRDMDDDLIAAHRDLPQLMPYLHLPVQSGSDRILAAMNRKHGRDDFFAIIEKMR QARPDMALSSDFIVGFPGESEKDFEDTLDLVRRVGFASAYSFKYSPRPGTPAADHDAQVP EEVKAERLAELQRLLEASKAAFDESCRGRTFDILLEKPGRQAGQLIGRSPYLQSVVVNDP PAAIGDLLTVTITDVGPNSLAGVPAEASEPSVSQSPVSSARSRPLAAMEA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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