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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP414270CWDYeast1mgQuote
EP414270CWDE. coli1mgQuote
BP414270CWDBaculovirus200ugQuote
MP414270CWDMammalian Cell200ugQuote

Protein Information

SpeciesCampylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A)
UniProt IDA7I0Q3
Gene NamemiaB; Locus:CHAB381_0506
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-429
Expression Tag6xHis
Purity>90%
AA SequenceMKLLYLQTLGCAMNVRDSEHIVAELKDEYKLTDDISKADLILINTCSVREKPVHKLFSEI GAFNKVRKNGSKIGVCGCTASHLGEEIFNKAPFVDFVLGARNVSKISEAVKTPKFISTDI NYDESEFAFGEFRGSPYKAFVNIMIGCDKKCSYCIVPQTRGKEISIPAEIILNEASKAVQ NGAKEIFLLGQNVNNYGKFFSSRHEKMDFSDLLVKISEIKGVERIRFTSPHPLHMDDKFL DIFTSNPKICKSMHMPLQSGSTKVLRDMRRGYDKEWFLNRALRLRKMCPEVSISTDIIVA YPTESEDDFKDTMEVLNEVKFEQIFSFKFSPRPLTAAENLPLIDNEIASKRLEILQSRHN EILDEIMKNQVGKIFDVYFEELRANGEVAGRSFSNFLVSVKGSEDLLGKILPVRIEKASR MVLYGKITA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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