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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP413024STMYeast1mgQuote
EP413024STME. coli1mgQuote
BP413024STMBaculovirus200ugQuote
MP413024STMMammalian Cell200ugQuote

Protein Information

SpeciesShewanella baltica (strain OS185)
UniProt IDA6WRJ1
Gene NamemiaB; Locus:Shew185_3303
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEASEADILLLNTCSIREKAQEKVF HQLGRWKTLKDKNPNLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIEQVRR GDKAVIDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPSDD IILEIAQLAEQGVREVNLLGQNVNAYRGATHDGAICTFAELLRFVAAIDGIDRIRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKAREGI QISSDFIIGFPGETKEDFADTMKLIEDIGFDHSFSFIYSARPGTPAADLPDNVDMEEKKQ RLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGQPKHIG TFVDVEIVDVYTNSLRGVFIRGEDEMDLRRSLRPAEILAKRKQDDELGVTQFKP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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