Request QuoteCatalog Number: xP425995BQDSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP425995BQDYeast1mgQuote
EP425995BQDE. coli1mgQuote
BP425995BQDBaculovirus200ugQuote
MP425995BQDMammalian Cell200ugQuote

Protein Information

SpeciesBacillus amyloliquefaciens (strain FZB42)
UniProt IDA7Z4X2
Gene NamemiaB; Locus:RBAM_016850
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-509
Expression Tag6xHis
Purity>90%
AA SequenceMNEKQKQESGQVNPADKTSEKDYSKYFEAVYIPPSLKDAKKRGKESVQYHNDFKISEEFK GLGEGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENK VFGELGHLKALKKNNPDLILGVCGCMSQEESVVNRILKKHPFVDMIFGTHNIHRLPELLS EAYLSKEMVIEVWSKEGDVIENLPKVRNGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR RPEEIIQEVRRLASEGYKEITLLGQNVNAYGKDFEDMTYGLGDLMDELRKIDIPRIRFTT SHPRDFDDHLIEVLAKGGNLLDHIHLPVQSGSSAMLKMMARKYDRERYLELVGKIKAAMP NASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVK KERLQRLNDLVKEISAKKMKEYEGRTVEVLVEGESKNNPDILAGYTEKSKLVNFKGPKDA IGKIVRVKIEQAKTWSLDGVMAGEAIEVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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