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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP414056CWWYeast1mgQuote
EP414056CWWE. coli1mgQuote
BP414056CWWBaculovirus200ugQuote
MP414056CWWMammalian Cell200ugQuote

Protein Information

SpeciesClostridium botulinum (strain Langeland / NCTC 10281 / Type F)
UniProt IDA7GE46
Gene NamemiaB; Locus:CLI_1796
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMNEILNTKDINTIGEFFIETWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCC VRENAELKVYGNLGILKGLKSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNL HNFPNYLNEVKKKDTSVLKIQEKEDSIIENMPIDRKNSMKAFVTIMYGCNNFCTYCIVPY VRGRERSRTPENIEAEIKKLISEGYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEG LERVRFMTSHPKDLTDDVIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVV SKIKKLIPNVALSTDIIVGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFE DQVPEDVKHKRFNRLVEVVNEISAKKNKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLV NFIGDKDSIGEFVNVKIIKANSFSLTGEEI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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