Request QuoteCatalog Number: xP425903FDYSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP425903FDYYeast1mgQuote
EP425903FDYE. coli1mgQuote
BP425903FDYBaculovirus200ugQuote
MP425903FDYMammalian Cell200ugQuote

Protein Information

SpeciesFrankia sp. (strain EAN1pec)
UniProt IDA8L6J8
Gene NamemiaB; Locus:Franean1_1226
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-494
Expression Tag6xHis
Purity>90%
AA SequenceMSGRSYQVRTFGCQMNVHDSERLAGLLESAGYVPAPEGSDADVVVFNTCAVRENADNRLY GNLGHLYPAKKSNPAMQIAVGGCLAQKDRSVILDRAPWVDVVFGTHNIHRLPVLLERARH NSAAQVEIAEALEVFPSSLPARRASHHSAWVSISVGCDNTCTFCIVPSLRGGERDRRPGD VLAEVEALVAEGALEITLLGQNVNSYGRSLGDPGAFAKLLRACGRVEGLERVRFTSPHPR DFTDDVIEAMAQTPNVCPQLHMPLQSGSDVVLRRMRRSYRRERFLGIVERVRAAMPDAAI TTDIIVGFPGETEADFADTLDVVRTARFAGAFTFKYSPRPGTPAAEMDGAVDPAVVSERY GRLASLQDEMSWAENRAQVGRRVEIVVAEGEGRKDSATGRLSGRARDGRLVHLLVTGPHA QEGLVRPGDVVETVVTRGAPHHLTADGPLLTHRRTRAGDAWAAGRAIAGDTPRPDRPAVS LGMPQLRPSAPAAR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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